Seeing through Murky Matter using Laser-induced Fluorescence A thesis submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Physics from the College of William and Mary in Virginia, by
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چکیده
Current techniques used to image gene expression in vivo use invasive surgical procedures or harmful gamma rays to analyze tissue. Optical imaging of tissue is difficult because the majority of light is absorbed by tissue. In the near infrared (NIR) region, however, absorption of light decreases and it is possible to “see” through tissue. Though absorption is minimal in the near infrared region, scattering due to tissue heterogeneities hinders optical imaging. We are developing a detector for laser-induced fluorescence in the NIR using a modulated avalanche photodiode (APD) to amplify the signal and the time delay of fluorescence to differentiate between scattered light and fluorescence. We have shown that by modulating the avalanche photodiode, we can amplify the signal by nearly 70%.
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Thesis Submitted in Partial Fulfillment of the requirement for the Degree of M.A/M. Sc In School consultant
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